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KS4 Tree of life

Give your key stage 4 pupils a challenge to investigate gene sequencing technology and evolutionary trees.

Challenge overview

Pupils will construct an evolutionary tree using their own classification system for the plants in your school grounds or nearby park/green space, then compare with data from Kew’s Plant and Fungal Trees of Life (PAFTOL). 

Learning outcomes 

  • Understand the importance and applications of Kew’s Plant and Fungal Trees of Life project 

  • Understand how the analysis of DNA sequence data has helped inform the modern classification system  

  • Understand that evidence for the modern theory of evolution by natural selection includes DNA sequence data 

  • Apply knowledge of sampling techniques to ensure a representative sample 

Key vocabulary 

Classification, genetics, genome, DNA, evolutionary tree, fieldwork, sampling, methodology 


Background information

How are the world’s plants and fungi related to each other and how have they evolved over time? Linnaeus’s original system of classification grouped organisms by physical characteristic – whether they looked similar or different. Since then, advances in technology mean that we can now classify plants by their DNA sequences. 

Find out how Kew’s Plant and Fungal Trees of Life (PAFTOL) project is using DNA sequencing technology to complete the evolutionary tree of life. 

Did you know? 

Kew’s Plant and Fungal Trees of Life project aims to generate DNA sequence data for at least one species from each of the approximately 14,000 flowering plant and 8,200 fungal genera.   

Tree of life main image

Wow fact 

PAFTOL scientists have sequenced hundreds of genes from herbarium samples that are over 200 years old! 

How to run the challenge

Part 1: Challenge preparation (1 hour) 

We recommend this is completed in class with the teacher.

  1. Introduce the challenge to your class by playing the short clip in the overview.  All the other tools needed for the challenge are in the 'Resources' section below

  2. Play the Watch and learn video with your class

  3. Print out the Odd One Out activity for your pupils to complete in groups, or display the cards on the whiteboard for a whole class approach 

  4. Print out the Taxonomic Categories resource for your pupils to read, or display the resource on the whiteboard for a whole class approach 

  5. Print out the supporting resources (“Indicator species guide” and “Sampling strategies”) for your pupils to use during the challenge 

  6. Print out the ‘From DNA sequences to trees’ resource for pupils to use after they’ve created their own tree 

  7. Assess your pupil's learning with the quiz

Part 2: The challenge (2-3 hours) 

Pupils can complete the challenge alone or in groups, and either in class or as a homework activity.

Kew’s Endeavour challenges are open-ended to encourage pupils to design and carry out their own investigations. For KS4 Tree of life, pupils must construct an evolutionary tree using their own classification system for the plants in your school grounds or nearby park/green space, then compare with the PAFTOL data. 

Pupils will need to: 

  • design a method for sampling an area 

  • look for the indicator species provided 

  • photograph or draw the species 

  • decide on a system of classification based on physical characteristics: 

    • what do the plants look like? 

    • can they be grouped by similarities and differences in appearance? 

  • construct an evolutionary tree for these plants using their classification system 

  • use the PAFTOL project’s Tree of Life to find out how closely the plants are related using gene sequence data and construct a new tree based on their DNA 

  • compare their two trees; how similar or different are they? 

  • write up their findings along with their ideas on why this work is important – why would we need to know how different plants are related to each other? 

Download lesson plan

Resources

KS4 Tree of life watch and learn

Introduce your pupils to gene sequencing technology and evolutionary trees with this

evolution and adaptation, conservation and sustainability
Watch and learn
KS 4

KS4 Tree of life odd one out

Invite your pupils to discover which plants are related to each other.

evolution and adaptation, conservation and sustainability
Activity
KS 4

KS4 Tree of life taxonomic categories

Invite your pupils to explore the science of naming, describing and classifying organisms

evolution and adaptation, conservation and sustainability
Infographic
KS 4

KS4 Tree of life indicator species guide

Help your pupils to complete a survey to find the indicator species with this

field studies, evolution and adaptation, conservation and sustainability
Infographic
KS 4

KS4 Tree of life sampling strategies guide

Find out about different field studies sampling strategies and methods.

field studies, evolution and adaptation, conservation and sustainability
Infographic
KS 4

KS4 Tree of life from DNA sequences to trees

Find out how DNA sequence data is used to construct evolutionary trees.

evolution and adaptation, conservation and sustainability
Activity
KS 4

KS4 Tree of life quiz

Test your pupils' knowledge of plant health with this quiz.

evolution and adaptation, conservation and sustainability
Quiz
KS 4

Career skills

Endeavour promotes the acquisition of Science Capital, helping your pupils to have a better understanding of science and its relevance in their lives. By taking this challenge your pupils will be using the skills of a bioinformatician. 

What does a bioinformatician do? 

There are many different jobs that a bioinformatician can perform. One of the jobs of a bioinformatician on the PAFTOL team is to develop and use computer programmes so that DNA sequence data can be assembled into evolutionary trees and analysed.

To do this, a bioinformatician analyses DNA sequences isolated from plant specimens by first aligning them to each other. The pattern of differences in the DNA base residues between each sequence are assessed by specially designed algorithms.

The output is visualized in the form of a tree showing the relationships between the sequences and therefore the plant species themselves (i.e. it is similar to a family tree of human relatives). These evolutionary trees help scientists to interpret the similarities and differences between related species with interesting characteristics. 

Skills: 

  • Observation - looking at things carefully 

  • Attentiveness - able to pay attention to detail 

  • Researching - finding relevant information 

  • Logic – basing thoughts on facts and evidence and using reasoning to solve problems 

  • Classifying - arranging, ordering and grouping things 

  • Questioning - identifying areas that can be studied or tested 

  • Inferring - making informed judgements 

  • Communication - written and verbal